Learn what "what game are you playing with my girls" means, when people say it, and how to use it naturally in English.

This means the speaker thinks the other person is being dishonest or manipulative with their daughters.
From The Dumb Billionaire Heiress In Love Part I, Episode 22
Someone accuses another person of playing games with their daughters.
Use it in a serious confrontation when you suspect bad intentions. It sounds hostile and is not appropriate in polite conversation.
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