Learn what "they will know not to trifle with me" means, when people say it, and how to use it naturally in English.

This means people should not treat the speaker lightly or challenge them carelessly.
From Two Worlds Apart, Episode 37
The speaker is warning others not to underestimate or mess with them.
Use this in a very serious or threatening context. It is not polite and can sound dramatic or intimidating.
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