Learn what "i know you're not my real sister" means, when people say it, and how to use it naturally in English.

This means the speaker is asserting that the other person is not a real or biological sister.
From Do Not Deceive Me, Episode 20
A speaker tells someone they know she is not their biological sister.
Use this to state a family relationship directly, usually in a tense conversation. It can sound harsh or emotionally loaded depending on context.
Scenes unlock real expressions as you watch
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