Learn what "end of the line lady" means, when people say it, and how to use it naturally in English.

It means this is the point where things stop for you or where you are finished.
From A Love Once Betrayed, Episode 27
The speaker is shutting someone down and saying their chance is over.
Use this in threatening or dramatic speech to mean someone has reached their limit. It sounds rude and old-fashioned in many contexts.
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